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. Author manuscript; available in PMC: 2019 Jan 17.
Published in final edited form as: J Am Chem Soc. 2017 Nov 10;139(46):16932–16938. doi: 10.1021/jacs.7b09932

Figure 3.

Figure 3

Expanded view of the CDMS spectrum showing the capsid peak at different times. The red trace shows the high mass range of the the assembly reaction. Each point represents a single ion. There are two clusters of ions with average charges centered around 140 and 155 e (elementary charges). Both clusters have masses around 4.1 MDa, though the lower charge cluster has a slightly broader distribution of masses and a slightly larger average mass. The higher charge cluster has around twice as many ions as the lower. Figure 4B shows the scatterplot for ions measured 24 h after initiation of the assembly reaction. At this time point, the low charge cluster has largely disappeared. Thus, the more highly charged cluster of ions that persists after 24 h is attributed to the T = 4 capsid.