Table 2.
Strains and plasmids used in this study.
| Strain or plasmid | Genotype or characteristic | Source |
|---|---|---|
| B. burgdorferi | ||
| B313 | High-passage B. burgdorferi B31 missing lp5, lp 17, lp21, lp25, lp28-1, lp28-2, lp28-3, lp28-4, lp36, lp38, lp54, lp56, cp9, cp32-4, cp32-6, cp32-8, cp32-9 | (Hallstrom et al., 2013) |
| B31-A3 | Clone of B. burgdorferi B31 missing cp9 | (Elias et al., 2002) |
| B31-A3ΔcspZ | B31-A3ΔcspZ::KanRa | (Coleman et al., 2008) |
| B31-A3ΔcspZ/pKFSS-1 | B31-A3ΔcspZ:: KanR carrying plasmid pKFSS-1 | This study |
| B31-A3ΔcspZ/pCspZ-WT | B31-A3ΔcspZ::KanR complemented with intact cspZ | This study |
| B31-A3ΔcspZ/pCspZ-Y207A/Y211A | B31-A3ΔcspZ::KanR complemented with intact cspZ-Y207A/Y211A | This study |
| E. coli | ||
| DH5α | F- Φ801acZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk−, mk+) phoA supE44 thi-1 gyrA96 re1A1 λ- | ThermoFisher |
| BL21(DE3) | F–, ompT hsdSB (rB– mB–) gal dcm (DE3) | Novagen |
| BL21 (DE3)/pGEX4T2-CspZ | BL21(DE3) producing GST-tagged residues 19 to 237 of CspZ | This study |
| BL21 (DE3)/pGEX4T2-CspZ-Y207A/Y211A | BL21(DE3) producing GST-tagged residues 19 to 237 of CspZ-Y207A/Y211A | This study |
| Plasmids | ||
| pJET 1.2/Blunt | AmpRb; PCR cloning vector | Therm oFisher |
| pGEX4T2 | AmpR; GST-tagged protein expression vector | Qiagen |
| pGEX4T2-CspZ | AmpR; pGEX4T2 encoding GST fusion protein residue 19 to 237 of CspZ | This study |
| pGEX4T2-CspZ-Y207A/Y211A | AmpR; pGEX4T2 encoding GST fusion protein residue 19 to 237 of CspZ-Y207A/Y211A | This study |
| pKFSS-1 | StrRc; Borrelia shuttle vector | (Frank et al., 2003) |
| pKFSS-1/pCspZ-WT | StrR; pKFSS-1 encoding intact cspZ | This study |
| pKFSS-1/pCspZ-Y207A/Y211A | StrR; pKFSS-1 encoding intact cspZ-Y207A/Y211A | This study |
a
Kanamycin resistant
b
Ampicillin resistant
c
Streptomycin resistant