Table 1.
Effects of DSS Colitis on Features of Trabecular Bone in Different Strains and Substrains of Mice
| C57BL/6 | Jackson | Taconic | |||||||||
| Vehicle (n=5) | 3% DSS (n=5) | Vehicle (n=10) | 4% DSS (n=7) | ||||||||
| Mean | ± SEM | Mean | ± SEM | Diff. | Mean | ± SEM | Mean | ± SEM | Diff. | ||
| Bone Volume Fraction (BV/TV; %) | 6.44 | ± 0.12 | 3.97 | ± 0.14 | **** | 3.56 | ± 0.14 | 1.17 | ± 0.07 | **** | |
| Trabecular Thickness (μm) | 40.7 | ± 1.8 | 36.3 | ± 1.1 | ns | 41.4 | ± 1.5 | 34.6 | ± 1.8 | ** | |
| Trabecular Number (1/mm) | 3.76 | ± 0.18 | 3.19 | ± 0.08 | * | 2.19 | ± 0.13 | 1.62 | ± 0.14 | * | |
| Trabecular Space (μm) | 267.7 | ± 13.7 | 313.3 | ± 7.6 | * | 477.5 | ± 28.5 | 656.5 | ± 65.2 | * | |
| Connective Density (1/mm3) | 51.5 | ± 1.5 | 26.7 | ± 1.3 | **** | 26.5 | ± 0.7 | 4.6 | ± 0.2 | **** | |
| Bone Density (mg HA/cm3) | 806.8 | ± 3.0 | 778.9 | ± 4.955 | ** | 787.2 | ± 6.2 | 760.0 | ± 11.4 | * | |
| Total Volume (TV; mm3) | 4.714 | ± 0.127 | 5.084 | ± 0.165 | ns | 6.189 | ± 0.175 | 6.719 | ± 0.307 | ns | |
| Bone Volume (BV; mm3) | 0.303 | ± 0.011 | 0.201 | ± 0.009 | **** | 0.220 | ± 0.010 | 0.080 | ± 0.007 | **** | |
| SMI | 2.87 | ± 0.09 | 3.05 | ± 0.01 | ns | 2.89 | ± 0.07 | 3.30 | ± 0.05 | *** | |
| Bone Surface (BS; mm2) | 19.44 | ± 0.46 | 13.95 | ± 0.23 | **** | 13.80 | ± 0.70 | 5.68 | ± 0.63 | **** | |
| BS/BV (1/mm) | 6.44 | ± 0.19 | 3.97 | ± 0.14 | ns | 69.10 | ± 2.70 | 83.30 | ± 3.28 | ** | |
| BALB/c | Jackson | Charles River | |||||||||
| Vehicle (n=5) | 5% DSS (n=5) | Vehicle (n=5) | 5% DSS (n=5) | ||||||||
| Mean | ± SEM | Mean | ± SEM | Diff. | Mean | ± SEM | Mean | ± SEM | Diff. | ||
| Bone Volume Fraction (BV/TV; %) | 8.50 | ± 0.29 | 4.32 | ± 0.19 | **** | 7.44 | ± 0.17 | 5.19 | ± 0.30 | *** | |
| Trabecular Thickness (μm) | 41.1 | ± 0.8 | 37.9 | ± 0.7 | * | 41.4 | ± 0.32 | 40.48 | ± 1.4 | ns | |
| Trabecular Number (1/mm) | 3.66 | ± 0.10 | 2.48 | ± 0.07 | **** | 3.30 | ± 0.09 | 2.58 | ± 0.10 | *** | |
| Trabecular Space (μm) | 275.1 | ± 7.8 | 409.3 | ± 12.1 | **** | 305.7 | ± 9.2 | 392.9 | ± 14.5 | *** | |
| Connective Density (1/mm3) | 114.1 | ± 4.4 | 41.0 | ± 2.4 | **** | 90.1 | ± 2.6 | 52.3 | ± 3.3 | **** | |
| Bone Density (mg HA/cm3) | 817.5 | ± 5.3 | 810.0 | ± 2.4 | ns | 819.9 | ± 4.0 | 798.4 | ± 4.7 | ** | |
| Total Volume (TV; mm3) | 3.250 | ± 0.070 | 3.440 | ± 0.070 | ns | 3.080 | ± 0.060 | 3.39 | ± 0.080 | * | |
| Bone Volume (BV; mm3) | 0.276 | ± 0.009 | 0.149 | ± 0.007 | **** | 0.227 | ± 0.004 | 0.175 | ± 0.007 | *** | |
| SMI | 2.46 | ± 0.02 | 2.79 | ± 0.04 | *** | 2.54 | ± 0.05 | 2.72 | ± 0.08 | ns | |
| Bone Surface (BS; mm2) | 17.04 | ± 0.49 | 9.67 | ± 0.32 | **** | 13.80 | ± 0.14 | 10.71 | ± 0.19 | **** | |
| BS/BV (1/mm) | 66.49 | ± 1.32 | 72.45 | ± 1.72 | * | 65.58 | ± 0.87 | 67.65 | ± 2.97 | ns | |
| SERT-/- on a C57BL/6 background | |||||||||||
| Vehicle (n=4) | 3% DSS (n=4) | ||||||||||
| Mean | ± SEM | Mean | ± SEM | Diff. | |||||||
| Bone Volume Fraction (BV/TV; %) | 2.48 | ± 0.14 | 2.06 | ± 0.02 | * | ||||||
| Trabecular Thickness (μm) | 36.1 | ± 2.2 | 32.7 | ± 1.3 | ns | ||||||
| Trabecular Number (1/mm) | 2.39 | ± 0.22 | 2.47 | ± 0.15 | ns | ||||||
| Trabecular Space (μm) | 432.3 | ± 38.2 | 409.6 | ± 24.5 | ns | ||||||
| Connective Density (1/mm3) | 13.73 | ± 1.07 | 8.78 | ± 0.67 | ** | ||||||
| Bone Density (mg HA/cm3) | 790.1 | ± 9.2 | 760 | ± 4.69 | * | ||||||
| Total Volume (TV; mm3) | 4.05 | ± 0.17 | 4.93 | ± 0.26 | * | ||||||
| Bone Volume (BV; mm3) | 0.100 | ±0.005 | 0.101 | ± 0.005 | ns | ||||||
| SMI | 3.24 | ±0.03 | 3.19 | ± 0.02 | ns | ||||||
| Bone Surface (BS; mm2) | 6.93 | ± 0.68 | 7.59 | ± 0.39 | ns | ||||||
| BS/BV (1/mm) | 79.32 | ± 5.45 | 87.13 | ± 4.41 | ns | ||||||
Trabecular bone μCT measurements of femurs of DSS-treated mice (initial concentration differed between strains) compared to controls (vehicle) with unpaired 2-tailed student’s t-test
*
P < 0.05
**
P < 0.01
***
P < 0.001
****
P < 0.0001
ns non-significantly different