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The fraction of antigen not released from microparticles in vitro during the soluble release phase can be accounted for via nitrogen analysis. Approximately 70% of encapsulated OVA was released as soluble antigen by day 35 from micropaticles. The remaining samples mass was found to contain approximately 27% of total encapsulated OVA, for 97% total recovery. ±SEM
| % Soluble OVA release (day 35) | % Remaining (N2 analysis) | Total OVA recovery 1 |
|---|---|---|
| 69.9% ± 0.0 | 26.9% ± 0.0 | 96.8% ± 3.5 |
