Figure 7.
IL-17A inhibition in WT mice recovered the phenotype observed in ΔdblGATA-1 and protected mice against A. fumigatus infection. WT mice were intranasally treated with anti-IL-17A Ab (5 μg per mouse) and infected via intranasal with 40 μL of suspension containing 1 × 108 conidia of A. fumigatus. (A) Lethality curve. After infection, mice were monitored for 7 days and comparative lethality curves were performed (at least 7 mice per group). All WT and ΔdblGATA-1 non-infected mice groups treated or not with anti-IL-17 antibody did not have lethality and were not shown. (B) Fungal burden. After 3 days of infection, homogenates from lungs were plated on YG-agar and colony-formit units were counted. (C–E) Inflammatory cell infiltrates determination. BALFs were harvested at 3 days after infection and (C) Total cells, (D) neutrophils and (E) macrophages were counted in BALF. Data are presented as Mean ± SD (n = 5 to 7 mice per group). *Significantly different (P < 0.05) between the same mice group.