Figure 3.

LNP‐mediated TNFα gene silencing accelerated wound healing in diabetic mice. (a) Mice were double‐wounded on Day 1 and treated on Days 2 and 3 with either PBS (control, left wounds) or LNPs containing siTNFα (250 nM, right wounds). Mice were sacrificed on Day 4. (b) The wound area is represented as a percent of its original size. By Day 4, nanoparticle‐treated wounds are statistically significantly smaller than PBS‐treated wounds. (c) Wounds treated with lipid nanoparticles containing siRNA specific against TNFα (siTNFα) experienced a 43% reduction in TNFα expression compared to PBS‐treated control wounds. In each panel, error bars represent s. d (n = 4–5, *p < .05, **p < .01)