Fig. 5. CALR-mutant-specific ex vivo responses in both CD4⁺ T_mem and T_naive compartments.

PBMCs from three healthy individuals (a, b, and c) were enriched for CD4⁺ T_mem and T_naive with MACS. Enriched cells were analyzed in an ex vivo IFN-γ ELISPOT. (Top) Photographs of wells stimulated with either CALRLong4 or a negative scrambled peptide; (middle) analysis of purity of the different cell fractions in CD3⁺ gated cells; (bottom) graph shows the number of cells specific for CALRLong1, CALRLong4, and CALRLong36. The number of peptide-specific cells was calculated by subtracting the number of spots in wells stimulated with a scrambled negative control peptide from the number of spots in peptide-stimulated wells. Error bars display standard error of the mean