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. 2019 Jan 17;9:164. doi: 10.1038/s41598-018-36631-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Study of collagen deposit following colonic anastomosis after irradiation using combined treatment (iterative injections). (A) Representative pictures of picrosirius staining 5 weeks after surgery thus 8 weeks after irradiation in the different groups of rats. Scale bars represent 200 µm. (B) Quantification of the ratio of the surface picrosirius positive staining to the total surface of the anastomosis scar. N = 5 animals per group Sham = control rat with surgery, IR = irradiated rat with surgery, IR + combined treatment = irradiated rat + surgery + combined treatment injected as described in the protocol 2 of Supplementary Fig. 2. ^**p < 0.01 vs. sham rats.