Table 2.
Major effects of heat priming regime in the main groups of metabolites.
| Super Pathway | Sub Pathway | Biochemical Name | TP2/TP1 | TP3/TP1 | TP4/TP1 | TP5/TP1 | TP5/TP4 |
|---|---|---|---|---|---|---|---|
| Amino acid | Aromatic amino acid metabolism (PEP derived) | 3-(4 hydroxyphenyl)lactate | 0.64 | 1.74 | 3.21**** | 1.01 | 0.32** |
| Glutamate family (alpha-ketoglutarate derived) | Trans-urocanate | 1.91 | 3.05 | 3.14 | 0.5** | 0.16* | |
| Cis-urocanate | 3.57 | 2.97*** | 3.84 | 0.87 | 0.23 | ||
| Glutathione metabolism | Ophthalmate | 0.73 | 2.36 | 3.96 | 0.47 | 0.12** | |
| Carbohydrate | TCA cycle | 2-methylcitrate | 0.93 | 2.25**** | 4.19**** | 5.17**** | 1.23 |
| Amino sugar and nucleotide sugar | UDP-N acetylglucosamine/galactosamine | 1.65 | 2.96*** | 3.69**** | 1.19 | 0.32** | |
| Sucrose, glucose, fructose metabolism | 1-kestose | 1.18 | 4.33**** | 10.78**** | 2.14**** | 0.2** | |
| Galactinol | 1.45 | 32.37**** | 56.6**** | 64.2**** | 1.13 | ||
| Raffinose | 0.77 | 15.51**** | 34.51**** | 10.35**** | 0.3** | ||
| Stachyose | 0.9 | 1.48 | 3*** | 14.21**** | 4.73**** | ||
| Sucrose | 0.29 | 10.95 | 45.69**** | 0.35 | 0.01** | ||
| Lipids | Fatty acid, Amino | 2-aminoheptanoate | 0.97 | 2.25*** | 3.07**** | 2.4*** | 0.78 |
| Phospholipid Metabolism | Glycerol 3-phosphate | 0.85 | 2.82*** | 4.44**** | 3.04*** | 0.69* | |
| Glycerophosphoethanolamine | 1.01 | 4.78*** | 4.57*** | 3.77 | 0.82 | ||
| Glycerophosphorylcholine (GPC) | 1.19 | 10.6**** | 15.14**** | 14.42**** | 0.95 | ||
| Glycerophosphoserine | 1.14 | 3.09**** | 3.15**** | 1.93**** | 0.61* | ||
| Lyso-galactolipids | 2-palmitoyl-galactosylglycerol (16:0) | 1.59 | 3.38 | 4.6*** | 3.66**** | 0.8 | |
| Nucleotide | Purine metabolism | Adenylosuccinate | 3.28 | 6.99**** | 3.74 | 0.34 | 0.09** |
Chart showing the significant changes caused by raising the temperature during the priming phase, only compounds increased by ≥3-fold at TP4/TP1 are shown. Two pathways are drastically affected, carbohydrate and lipid, showing dramatic increases especially in the sub-pathways associated with sucrose and phospholipid metabolism. Data key: ****significant difference (p ≤ 0.05) between the groups shown, metabolite ratio of ≥1.00; ***narrowly missed statistical cutoff for significance 0.05 < p < 0.10, metabolite ratio of ≥1.00; **significant difference (p ≤ 0.05) between the groups shown, metabolite ratio of <1.00; *narrowly missed statistical cutoff for low significance 0.05 < p < 0.10, metabolite ratio of <1.00, No asterisks = no significance, showing metabolite ratio that missed statistical cutoff of p = 0.10 between the groups shown.