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. 2019 Jan 17;9:197. doi: 10.1038/s41598-018-36560-4

Figure 1.

Figure 1

Chromobox 6 (CBX6) is downregulated in breast cancer. (A) mRNA expression of CBX6 in breast cancer versus normal breast tissues. The data are retrieved from the RNA-Seq datasets of The Cancer Genome Atlas (TCGA). The expression values are presented as Reads Per Kilobase of transcript per Million mapped reads (RPKM). An unpaired two-tailed t-test was used to evaluate significant differences. P values are indicated at the top of the graph. (B) CBX6 expression in human non-tumorigenic breast epithelial (MCF-10A) and two human breast adenocarcinoma (MCF-7 and MDA-MB-231) cell lines. Left: qRT-PCR analysis of CBX6 mRNA level in MCF-10A, MCF-7 and MDA-MB-231 cells. GAPDH was used for normalization of expression. Data are presented as mean ± S.D. from three independent experiments performed in triplicate. *P < 0.05. Right: Western blot analysis of CBX6 protein levels in MCF-10A, MCF-7 and MDA-MB-231 cells with GAPDH as a loading control. (C) Kaplan-Meier analysis of overall survival of breast cancer patients with different levels of CBX6 expression. Patient survival and gene expression data were downloaded from TCGA’s data portal and patients were split into two groups with the median CBX6 mRNA expression level as the cut-off.