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. 2019 Jan 17;9:197. doi: 10.1038/s41598-018-36560-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CBX6 directly downregulates the expression of Bone Marrow Stromal cell antigen 2 (BST2). (A) CBX6 downregulates BST2 expression in MCF-7 cells. Left: qRT-PCR analysis of BST2 mRNA in the control (empty vector) and CBX6-overexpressing (CBX6-OE) cells using GAPDH for normalization. Data are presented as means ± S.D. from three independent experiments performed in triplicate. *P < 0.05. Right: Western blot analysis of BST2 in control (empty vector) and CBX6-overexpressing cells (CBX6-OE) using GAPDH as a loading control. (B) CBX6 downregulates BST2 expression in U251MG cells. qRT-PCR analysis of BST2 mRNA levels in control (empty vector) and CBX6-overexpressing cells using GAPDH for normalization. Data are presented as means ± S.D. from three independent experiments performed in triplicate. *P < 0.05. (C) Enrichment of CBX6 and H3K27me3 at the BST2 promoter detected via ChIP-qPCR. Top: The locations of primers at the BST2 promoter. Bottom: Enrichment of CBX6 and H3K27me3 at the BST2 promoter in control (empty vector) and CBX6-overexpressing cells. Data are presented as a percentage of the input, and as means ± S.D. from three independent experiments performed in triplicate. *P < 0.05. (D) Spearman’s rank correlation coefficient indicating a negative correlation between CBX6 and BST2 mRNA levels in breast cancer tissues based on The Cancer Genome Atlas (TCGA) RNA-Seq data.