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. 2019 Jan 17;9:193. doi: 10.1038/s41598-018-36969-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LPS-induced responses of various ex vivo primary cell cultures derived from the hTNF.LucBAC mouse strain. Luciferase activity as detected in peritoneal macrophages (a) or splenocytes (b) without stimulation (dotted line) or after stimulation (solid line) with LPS (10 ng/mL); data are representative of three independent experiments, with triplicate cultures per experiment (N = 3, n = 3). Tnf and luciferase mRNA abundance as detected in LPS-stimulated peritoneal macrophages (c) and splenocytes (d) over 6 h (N = 3 mice). Soluble TNF released to the culture medium in response to LPS treatment of peritoneal macrophages (e) and splenocytes (f) over 24 h (N = 6 mice). Bars represent standard error of the mean. Significant differences from unstimulated controls, *p < 0.05, **p < 0.01, ***p < 0.001; ANOVA.