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. 2019 Jan 17;9:193. doi: 10.1038/s41598-018-36969-x

Figure 6.

Figure 6

Effect of signal pathway inhibitors on LPS-induced luciferase activity in hTNF.LucBAC BMDMs. Cells left untreated (dotted line) or were stimulated with 10 ng/mL LPS in absence (black line) or presence of MEK inhibitor PD0325901 (PD; blue), proteasome inhibitor MG132 (MG; green) and IKK-2 inhibitor BI605906 (BI; dotted red) (a). Luciferase activity represented as area under the curve (AUC) in the absence or presence of each inhibitor in LPS-induced cells (b). Soluble TNF in the supernatants of the same cultures, 24 h post-stimulated or untreated BMDMs, in the absence or presence of each inhibitor. TNF levels were undetectable in the presence of each inhibitor alone (c). Data are representative of three independent experiments, with triplicate cultures per experiment (N = 3, n = 3) and bars represent standard error of the mean. Significant differences compared to LPS stimulated cells, **p < 0.01, ***p < 0.001; ANOVA.