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. 2019 Jan 17;9:160. doi: 10.1038/s41598-018-37232-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Altered expression of Panx1 during lethal sepsis. Balb/c mice were subjected to lethal sepsis by a surgical procedure termed cecal ligation and puncture (CLP), blood and various tissues were harvested at 24 h post CLP to measure Panx1 protein (Panel a,b) or mRNA levels (Panel c) by Western blotting and real-time RT-PCR, respectively. Serum Panx1 levels were measured by the optical intensity of the 48-kDa band on the Western blot, and expressed as the % of the optical band intensity of corresponding total proteins on SDS-PAGE gels. *P < 0.05 versus normal “−CLP” control.