Skip to main content
. 2019 Jan 17;9:160. doi: 10.1038/s41598-018-37232-z

Figure 5.

Figure 5

Panx1 mimetic peptide 10Panx enhanced LPS-induced macrophage hemichannel activation. (a,b) 10Panx elevated the LPS-induced Lucifer Yellow dye uptake. RAW264.7 cells were stimulated with LPS (1.0 μg/ml) in the absence or presence of 10Panx at indicated concentrations for 16 h, and subsequently incubated with Lucifer Yellow (LY, 1.0 mg/ml) for 15 min. Following fixation and three extensive washes, the number of cells with diffused fluorescent signals was counted under a fluorescence microscope, and expressed as a percentage of total cell numbers (DAPI-stained nuclei) in six fields. (c) 10Panx enhanced the LPS-induced ATP release. RAW264.7 cells were cultured in serum-free DMEM medium, and stimulated with LPS (1.0 μg/ml) in the absence or presence of Panx1 mimetic peptide at indicated concentrations, and the cell-conditioned culture medium was collected and subjected to ATP measurement. *P < 0.05 versus “-LPS” Control; #P < 0.05 versus “+LPS” control.