Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 17;9:198. doi: 10.1038/s41598-018-36618-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

CRY1/2 and FBXL3 affect TLK2 stability. (A,C) Proteins detected by IB in WCL or following FLAG IP from 293 T cells expressing the indicated plasmids and following treatment with CHX for the indicated times. In (C) shaded triangles indicate increasing HA-CRY1 or HA-CRY2 expression. * denotes non-specific signal. (B) Quantitation of IB data from three experiments as shown in (A). Each data point represents the mean ± s.e.m. of the signal for FLAG-TLK2 overexpressed alone (black) or with overexpressed FBXL3 + CRY2 (gray) divided by the signal for βACTIN from the same sample, normalized to the sample collected before addition of CHX (t = 0). The dotted lines represent the best exponential fit to each data set, from which the half-lives were calculated according to the formula t_1/2 = Tln(2), where T is derived from the exponential fit equation N(t) = N0exp(−t/T). **P < 0.01 by two-way ANOVA.