Figure 2.

Characterization of the tupanvirus replication cycle in A. castellanii by IF. A. castellanii monolayer was infected by tupanvirus at an M.O.I. of 10 and visualized by IF. At early timepoints, tupanvirus particles are observed attaching to the amoebae surfaces, even after th cell washing step was done. The VF forms between 8 and 12 h.p.i. At 16 and 24 h.p.i. the A. castellanii cytoplasm is filled with viral particles and cell lysis is observed at 24 h.p.i. (at which time the cytoskeleton of lysed amoebas is visualized, although these cells remain adhered). The viral particles are in green (anti-tupan particle antibody) and amoeba cytoskeleton in red (stained by Evans Blue). Scale bar, 100 µm.