Fig. 2.

IGF-II promoted differentiation of subcutaneous but not visceral preadipocytes. Subcutaneous and visceral preadipocytes were differentiated for 14 days in the presence or absence of IGF-II at 7.5 ng/ml or 62.5 ng/ml. A: micrograph of differentiated preadipocytes stained in Oil Red O at day 14 of differentiation. Magnification at (×10). B: quantitative Oil Red O staining absorbance analysis of A showing a reduction in Oil Red O absorbance in visceral adipocytes (P < 0.05) and an increase in absorbance in subcutaneous differentiated adipocytes (P < 0.01) with IGF-II treatment (62.5 ng/ml). C: Western blot of the differentiation markers PPARγ and adiponectin. D: densitometry of adiponectin. E: densitometry of PPARγ Western blot showing protein abundance after normalization to the reference protein β-actin. Data expressed as means ± SE of duplicate runs for absorbance analysis; each Western blot densitometry is representative of experiments performed in triplicate from three individual biopsies (n = 3). Statistical analysis performed using one-way ANOVA on day 14 of differentiation (*P < 0.05, **P < 0.01).