Fig. 3.

IGF-II treatment reduced fat deposition in visceral adipocytes after 14 days of differentiation. Western blotting of paired subcutaneous and visceral preadipocytes differentiated for 14 days with IGF-II (7.5 ng/ml or 62.5 ng/ml) showing insulin receptor, GLUT4, and FASN protein abundance (A). Densitometry analysis of insulin receptor protein abundance (B) GLUT4 (C), and FASN (D). β-Actin was used to ensure equal loading of samples. Densitometry represents the means ± SE of three experimental repeats from three individual biopsies (n = 3). Insulin stimulated H³ −2deoxy glucose uptake for visceral and subcutaneous adipocytes following differentiation with IGF-II (n = 3) (E). Statistical analysis performed using one-way ANOVA on day 14 of differentiation (*P < 0.05, **P < 0.01, ***P < 0.001). FASN, fatty acid synthase; GLUT, glucose transporter; IR, insulin receptor.