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. 2018 Jul 24;315(6):E1098–E1107. doi: 10.1152/ajpendo.00409.2017

Fig. 4.

Fig. 4.

Effect of IGF-II treatment on receptor expression in differentiated adipocytes. A: relative mRNA expression of the insulin receptor (IR). B: IGF-IR following 24 h IGF-II (62.5 ng/ml) treatment was performed using qPCR showing downregulation of the IR in visceral adipocytes. Insulin receptor isoform distribution in preadipocytes (C) and adipocytes (D). Relative mRNA expression of the insulin receptor isoforms IR-A and IR-B in subcutaneous (E) and visceral (F) adipocytes showing downregulation of IR-A and IR-B with IGF-II treatment in visceral adipocytes and in IR-B expression in subcutaneous adipocytes. In all experiments, expression was normalized to the reference gene GAPDH; data represented as the means ± SE of experiments performed in duplicate runs from individual biopsies (n = 6–4). Statistical analysis performed using one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.001).