Fig. 5.

Effect of IGF-II treatment on GLUT 4 and glucose uptake in differentiated adipocytes. A: relative mRNA expression levels of GLUT4 in subcutaneous and visceral adipocytes measured by qPCR and normalized to GAPDH reference gene. B: Western blot showing GLUT4 protein abundance; GAPDH used a loading control. C: densitometry analysis of B indicating a reduction in GLUT4 protein abundance in visceral adipocytes. D: [³H]2-Deoxy glucose uptake following IGF-II treatment in visceral and subcutaneous adipocytes. Data expressed as the means ± SE of duplicate runs for qPCR; each Western blot densitometry is representative of experiments performed in triplicate from three individual biopsies (n = 3). Statistical analysis performed using one-way ANOVA (*P < 0.05, ***P < 0.001). GLUT, glucose transporter.