Fig. 3.

miR-21 genetic ablation exacerbates ALDO/SALT-mediated LV fibrosis. miR21KO or WT mice were treated with ALDO/SALT or vehicle for 2 or 8 wk, and LV gene expression was quantified by RT-qPCR (A–E). miR21KO or WT mice were treated with ALDO/SALT or vehicle for 8 wk, and cardiac interstitial fibrosis was quantified in Picro Sirius Red-stained sections (F). Results are means ± SE (n = 6–8). *P < 0.05. ALDO, aldosterone; AU, arbitrary unit; Col1a1, collagen I; Col3a1, collagen III; Ctgf, connective tissue growth factor; Fn1, fibronectin 1; Lox, lysyl oxidase; LV, left ventricle; miR-21, microRNA-21; miR21KO, miR-21 knockout; WT, wild type.