Fig. 6.

miR-21 genetic ablation exacerbates ALDO/SALT-mediated cardiac dysfunction without affecting hypertension. miR21KO or WT mice were treated with ALDO/SALT or vehicle for 8 wk. Ejection fraction (A), fractional shortening (B), and cardiac output (C) were measured by echocardiography. Each determination is the mean of 12–15 cardiac cycles derived from 3 separate M-Mode images during each session. Blood pressure was measured by means of an indwelling catheter in freely moving conscious animals (D). Results are means ± SE (n = 5). *P < 0.05. ALDO, aldosterone; miR-21, microRNA-21; miR21KO, miR-21 knockout; NS, not significant; WT, wild type.