Fig. 2.
Adipocyte-specific thrombospondin 1 (TSP1) deficiency did not protect mice from high-fat diet-induced obesity, inflammation, or insulin resistance. Male TSP1∆adipo mice and control TSP1fl/fl (8 wk old) were fed with low-fat (LF) or high-fat (HF) diet for 32 wk. A: weekly body weight. B: fat and lean mass of mice measured by EchoMRI before the end of the study. C and D: ip glucose tolerance test (C) and insulin sensitivity test (D) were performed in mice at the end of study. E: expression of proinflammatory cytokines, macrophage markers, and TSP1 in epididymal adipose tissue (EAT) was determined by real-time PCR and normalized to 18S RNA. F: plasma TNF-α, IL-1β, MCP-1, and TSP1 levels measured by ELISA. Data are presented as means ± SE (n = 5–9 mice/group).