Fig. 3.

Myeloid/macrophage-specific thrombospondin 1 (TSP1) deficiency did not protect mice from diet-induced obesity, but high-fat (HF)-fed TSP1^{∆ mɸ} mice had improved glucose tolerance and insulin sensitivity. Male TSP1^∆Mɸ mice and control TSP1^fl/fl mice (8 wk old) were fed with low-fat (LF) or HF diet for 32 wk. A: weekly body weight. B: fat and lean mass of mice measured by EchoMRI before the end of the study. C: plasma insulin levels measured by ELISA. D and E: ip glucose tolerance test (D) and insulin sensitivity test (E) were performed before the end of the study, and area under curve (AUC) was calculated as described in materials and methods. Data are presented as means ± SE (n = 5–10 mice/group). **P < 0.01, ***P < 0.001, and ****P < 0.0001 (2-way ANOVA).