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. 2018 Aug 31;315(6):H1835–H1850. doi: 10.1152/ajpheart.00293.2018

Fig. 3.

Fig. 3.

Smooth muscle Jagged1 deficiency blunts arterial force production without altering vasorelaxation. A: impaired aortic (Ao) constrictor response to depolarization (KCl, left) or agonist constrictor [phenylephrine (PE), right] with Jagged1 deficiency [tamoxifen (TM) treatment (+TM)] versus controls (−TM). Myography data represent means ± SE (n = 4). Data for KCl were analyzed using a t-test, *P = 0.015; data for PE were analyzed using two-way ANOVA with Bonferroni’s post hoc test, *P < 0.05 and **P < 0.01. B: impaired mesenteric artery (MA) force production in response to depolarization (KCl, left) or agonist (PE, right) with Jagged1 deficiency (+TM) versus controls (−TM). Myography data represent means ± SE (n = 5). Data for KCl were analyzed using a t-test, *P = 0.014; data for PE were analyzed using two-way ANOVA with Bonferroni’s post hoc test, *P < 0.05. C: representative immunoblots showing blunted phosphorylated myosin light chain (p-MLC) production in aortic segments from Jagged1-deficient animals (+TM) versus controls (−TM) treated for 15 min with constrictor [KCl or angiotensin II (ANG II)]. Data represent means ± SE (n = 3) normalized to −TM and were analyzed using a t-test, *P = 0.002 and **P = 0.017. T-MLC, total MLC; P/T-MLC, p-MLC-to-total MLC ratio. D: preserved mesenteric artery (MA) relaxation responses to sodium nitroprusside (SNP, left) and acetylcholine (ACh, right) with Jagged1 deficiency (+TM) versus controls (−TM). Myography data represent means ± SE (n = 5) and were analyzed using two-way ANOVA with Bonferroni’s post hoc test. Max, maximum. E: Ca2+ permeabilization and force generation experiments. MAs from control and TM-treated smooth muscle myosin heavy chain-CreERT2 (SMMHC-CreERT2)/Jagged1 flox/flox (Jag1f/f) mice were permeabilized with α-toxin and exposed to incremental Ca2+ concentrations. Myograph data are expressed as tension generated above set baseline at pCa 9. Data represent means ± SE (n = 4 per group) and were analyzed using two-way ANOVA with Bonferroni’s post hoc test, *P < 0.05 and **P < 0.001.