Table 3.

Effect of DHEA and GDP-β-S on parameters of f_∞-V and f_∞-HP relationships

	n	V0.5, mV	k, mV
Control, f∞-V	15	−30.3 (0.5)	7.5 (0.4)
30 µM DHEA	15	−36.2 (0.5)*	7.2 (0.5)
Control, f∞-HP	9~15	−41.8 (0.1)	6.0 (0.1)
10 µM DHEA	9~15	−43.4 (0.5)*	6.4 (0.4)
30 µM DHEA	9~15	−46.3 (0.5)*	6.3 (0.5)
100 µM DHEA	9~15	−52.4 (0.7)*	6.5 (0.9)
Control, f∞-V	15	−28.9 (0.5)	7.7 (0.4)
GDP-β-S, f∞-V	7	−23.1 (0.5)*	7.9 (0.4)

Mean (SD) values were obtained by fitting plot with 5 points for holding potential (HP)-dependent steady-state inactivation curves (f_∞-HP) and 13 points for quasi-steady-state inactivation curves (f_∞-V); n, number of cells. V_0.5, voltage that causes a 50% reduction in the amplitude of the inactivating component of L-type Ca²⁺ current; k, slope factor. Statistical comparison in the absence and presence of dehydroepiandrosterone (DHEA) and control versus guanosine 5′-O-(2-thiodiphosphate) (GDP-β-S) was performed using ordinary one-way ANOVA followed by Dunnet’s test.

^*P < 0.001 compared with control.