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. 2018 Oct 17;315(6):F1695–F1707. doi: 10.1152/ajprenal.00246.2018

Fig. 7.

Fig. 7.

Periostin regulates genes involved in integrin/ECM signaling in autosomal dominant polycystic kidney disease (ADPKD) cells. Human ADPKD cells (n = 3 or 4 kidneys) were grown as polarized monolayers on permeable Transwells and incubated with control media (Cont) or 250 ng/ml recombinant periostin (Postn) for 24 h. Total RNA was isolated and reverse transcribed, and mRNA levels for the genes of interest were detected using quantitative RT-PCR and normalized to GAPDH. Bar graphs are expressed as means ± SE for relative changes in the expression of αV-integrin (ITGAV) (A), type I collagen (COL1A1) (B), type II collagen (COL2A1) (C), β-actin (ACTB) (D), filamin-A (FLNA) (E) and vinculin (VCL) (F). These genes were selected since they were found to be altered by periostin in a Bio-Rad PrimePCR array (Table 1). *P < 0.05 and **P < 0.01, compared with Cont.