Fig. 9.

Periostin promotes the migration of autosomal dominant polycystic kidney disease (ADPKD) cells. A: ADPKD cells were seeded into individual wells of 12-well plates containing media + 1% FBS. Serum was reduced to 0.05% for 24 h, and a scratch in each monolayer was made using a 200-µm pipette tip. Wounded monolayers were incubated in control media (A and B) or media containing 250 ng/ml periostin (C and D), and images of the monolayers were taken at 4 h (A and C) and 8 h (B and D). Bar graphs (means ± SE) represent the quantification of cell migration (wound closure) between 4 and 8 h after wounding for ADPKD (n = 6) (E) and normal human kidney (NHK; n = 3) (F) cells in Cont, periostin (Postn) or 40 ng/ml hepatocyte growth factor (HGF), a positive control for promoting cell migration (50). **P < 0.01, *P < 0.05, compared with Cont.