Fig. 10.

Periostin stimulates focal adhesion kinase (FAK) and Rho-mediated cytoskeletal changes in autosomal dominant polycystic kidney disease (ADPKD) cells. A: human ADPKD cells were incubated in media containing 250 ng/ml Postn for 30 to 120 min. Immunoblot analysis was used to measure levels of phosphorylated FAK (P-FAK) and total FAK. In the representative immunoblot, splice marks indicate the removal of extraneous lanes within the same blot. Bar graph (means ± SE) represents P-FAK/FAK. B: Rho was immunoprecipitated from cell lysates using GST-RBD beads with a high affinity for GTP-bound Rho. For a negative control, GDP was added to the lysate to inactivate endogenous Rho before immunoprecipitation (lane 1). GTP-Rho from the immunoprecipitate and total Rho from whole cell lysates were detected by immunoblotting using a Rho antibody. Bar graph represents GTP-bound Rho/total Rho. **P < 0.01, compared with Control (Cont; set to 1.0).