Table 1.
Effect of periostin on gene expression in ADPKD cells
| Gene Name | Description | Fold Change |
|---|---|---|
| ECM | ||
| COL1A1 | collagen I α1 | 4.0 |
| COL4A4 | collagen IV α4 | 1.8 |
| Cytoskeleton | ||
| FLNA | filamin A | 2.1 |
| ACTB | β-actin | 2.0 |
| ACTN1 | actinin α1 | 1.7 |
| ACTN4 | actinin α4 | 1.6 |
| Integrins | ||
| ITGAV | αV-integrin | 2.0 |
| ITGA5 | α5-integrin | 1.5 |
| Focal adhesion-integrin signaling | ||
| AKT1 | AKT serine/threonine kinase | 1.6 |
| ILK | integrin-linked kinase | 1.6 |
| Growth factor signaling | ||
| SRC | Src protooncogene | 2.5 |
Human primary autosomal dominant polycystic kidney disease (ADPKD) cells (n = 3 kidney preparations) were grown on Transwells to form polarized epithelial monolayers. Cells were treated with or without periostin for 24 h. Total cellular RNA was isolated and reverse transcribed. mRNA levels of 220 genes involved integrin outside-in signaling were detected using pre-designed PrimePCR arrays. The results were analyzed using Bio-Rad CFX Manager software. Genes that showed a greater than ± 1.5-fold significant change in gene regulation with P < 0.05, compared with control, are represented here.