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Identification of TMEM16A mRNA in renal microvessels by real-time PCR (A), and the relative abundance of TMEM16A between a Sprague-Dawley rat and a spontaneous hypertensive rat (SHR) (B). Immunofluorescence of TMEM16A (green) and α-actin (red) in renal vascular smooth muscle cells on a partially digested renal arteriole (C). Scale bar is 30 µm. *Significant difference (n = 8, P < 0.05).
