Fig. 4.

Analysis of renal fibrosis. A: Picrosirius red staining of the kidney. Shown are representative images from 6 rats/group. B: quantitative reverse transcription-polymerase chain reaction (q-RT-PCR) analysis of renal mRNA expression of transforming growth factor-β1 (TGF-β1) with normalization by β-actin. C–E: immunoblotting analysis of TGF-β1, collagen 1 (COL-1), and fibronectin (FN) in the cortex. Shown are representative blots with densitometry data. β-Actin was used as an internal control. Data are means ± SE; n = 6/group. *P < 0.05 vs. control (CTR); #P < 0.05 vs. albumin overload (AO).