Fig. 1.

Nfil3^−/− mice are grossly deficient in natural killer (NK) and ILC1 cells. A: representative flow cytometry plots showing the gating of CD3⁻NK1.1⁺NKp46⁺ NK cells within the circulation of 8–10-wk-old wild-type (WT) and Nfil3^−/− mice. B: absolute quantification of circulating NK1.1⁺NKp46⁺ NK cells (n = 7). C and D: representative flow cytometry plots showing the gating of NK and ILC1 populations within the lungs of WT and Nfil3^−/− mice. E: quantification of lung conventional NK (cNK) cells. F: quantification of lung CD49a⁺DX5⁻ ILC1 cells (n = 7). Statistical significance was determined using the Mann-Whitney test. *P < 0.05, **P < 0.01, and ***P < 0.001. Means ± SE. ILC, innate lymphoid cell.