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. Author manuscript; available in PMC: 2019 Jul 24.
Published in final edited form as: Lab Chip. 2018 Jul 24;18(15):2202–2213. doi: 10.1039/c8lc00332g

Table 1.

Media Conditions

Name First 2 days Last 2 days
EM/EM EM in the luminal / EM in the basal EM in the luminal / EM in the basal
DM/EM EM in the luminal / EM in the basal DM in the luminal / EM in the basal
DM/DM EM in the luminal / EM in the basal DM in the luminal / DM in the basal

EM: expansion medium with the growth factors (Wnt-3A, R-spondin, Noggin)

DM: differentiation medium without the growth factors