Table 4.
Resolution (Rs) quantification between HT (2-(3,4-dihydroxyphenyl)ethanol) and T (2-(4-hydroxyphenyl)ethanol) for different changes in the HPLC method: flow rate, wavelength, and gradient method, using the Kinetex biphenyl column.
| Method Changes | Rs (HT-T) ± SD |
|---|---|
| No change | 5.9 ± 0.2 |
| Flow rate = 1.4 mL/min | 5.91± 0.03 |
| Flow rate = 1.6 mL/min | 4.99 ± 0.06 |
| Wavelength = 278 nm | 5.6 ± 0.2 |
| Wavelength = 282 nm | 5.7 ± 0.2 |
| Gradient method = −2% for eluent B | 5.0 ± 0.1 |
| Gradient method = +2% for eluent B | 6.15 ± 0.05 |