Table A2.
Relation between capacity factor (k) and log Pow of reference compounds in phosphate buffer.
| pH | Linear Equitation | r2 | Quadratic Equitation | r2 |
|---|---|---|---|---|
| 4.5 | y = 15.564x − 9.2141 | 0.8970 | y = −7.6607x2 + 54.46x − 55.269 | 0.9953 |
| 5.5 | y = 14.935x − 6.5672 | 0.8942 | y = −7.5071x2 + 53.051x − 51.698 | 0.9964 |
| 6.5 | y = 14.855x − 6.4229 | 0.8965 | y = −7.3626x2 + 52.238x − 50.685 | 0.9962 |
| 7.5 | y = 14.895x − 6.2264 | 0.8924 | y = −7.5201x2 + 53.077x − 51.435 | 0.9954 |
| 8.5 | y = 14.99x − 6.7367 | 0.8925 | y = −7.5849x2 + 53.501x − 52.335 | 0.9959 |
The separation of reference compounds was carried out in 5 mM phosphate buffer with MeOH (5%, v/v), pH was set in the range of 4.5–8.5 (HPLC mobile phase A) in a linear gradient with MeOH (100%; HPLC mobile phase B) at 25 °C. For further conditions, see Materials and Methods section.