Figure 3.

Prohibitin is post-translationally deiminated in GBM cells and affected by PAD-inhibitor Cl-amidine. (A) PHB protein is present in both GBM cell lines. (B) The presence of deiminated PHB is verified in both LN18 and LN229 cells, as assessed by probing the PHB antibody on F95-immunoprecipitated protein eluates (IP:F95). (C) PHB protein levels are reduced in LN18 and LN229 after 1 h treatment with Cl-amidine. (D) Post-translational deimination of PHB is reduced in GBM cells following 1 h Cl-amidine treatment. R represents relative densitometry compared to β-actin, which was used as the internal loading control.