Table 2.
Polymerase chain reaction (PCR) primers.
| Name of Primers | Sequence (5′–3′) |
|---|---|
| cDNA Cloning of Three Cathepsin L-like Cysteine Proteinase Genes | |
| 3′ RACE (rapid amplification of cDNA ends) outer primer | TACCGTCGTTCCACTAGTGATTT |
| 3′ RACE inner primer | CGCGGATCCTCCACTAGTGATTTCACTATAGG |
| GSP (gene specific primer) 1-1 | GCAATGGTGGACTTATGGAC |
| GSP2-1 | AATCCAAGAGCCCCGTTATC |
| GSP3-1 | GCACCTACCGAAGCCGATACTA |
| GSP3-2 | CCACTCCAAGACTACCAAGG |
| 5′RACE outer primer | CATGGCTACATGCTGACAGCCTA |
| 5′RACE inner primer | CGCGGATCCACAGCCTACTGATGATCAGTCGATG |
| GSP1-2 | CTTGACGATCCAGTAGTCGC |
| GSP1-3 | CTCGCCATTTGGTCGCATTT |
| GSP2-2 | GGTTCTATCGCCGACATTCT |
| GSP3-3 | AACCAAAGTGTAGCCCCAAT |
| GSP3-4 | TGACCAAAGCGTTGCGAAGT |
| M13F(−47) | CGCCAGGGTTTTCCCAGTCACGAC |
| M13R(−48) | AGCGGATAACAATTTCACACAGGA |
| Preparation of Template DNA for ISH | |
| I-Bx-cpl-1-F | CCTTTCGCTGAATACCGTCGTCTTA |
| I-Bx-cpl-1-R | TGATGACTCAAGCCAGCGGATAACT |
| I-Bx-cpl-1-T7-F | TAATACGACTCACTATAGGGCCTTTCGCTGAATACCGTCGTCTTA |
| I-Bx-cpl-1-T7-R | TAATACGACTCACTATAGGGTGATGACTCAAGCCAGCGGATAACT |
| I-Bx-cpl-2-F | GCTGTGGATGTTGCTACGCTTTTGC |
| I-Bx-cpl-2-R | GCTTCTCCGTAGTCCTCTCCCCATT |
| I-Bx-cpl-2-T7-F | TAATACGACTCACTATAGGGGCTGTGGATGTTGCTACGCTTTTGC |
| I-Bx-cpl-2-T7-R | TAATACGACTCACTATAGGGGCTTCTCCGTAGTCCTCTCCCCATT |
| I-Bx-cpl-3-F | ACAGCAGTGCCAAGCCCGCTCAAAT |
| I-Bx-cpl-3-R | GTGCTCGGGCATTGATGATTCCTCC |
| I-Bx-cpl-3-T7-F | TAATACGACTCACTATAGGGACAGCAGTGCCAAGCCCGCTCAAAT |
| I-Bx-cpl-3-T7-R | TAATACGACTCACTATAGGGGTGCTCGGGCATTGATGATTCCTCC |
| Preparation of Template DNA for dsRNA | |
| Bx-cpl-1-T7-F | GCCAGTCGTCATCACAAA |
| Bx-cpl-1-R | TGTTCCTCATCGGCTTCT |
| Bx-cpl-1-F | TAATACGACTCACTATAGGGGCCAGTCGTCATCACAAA |
| Bx-cpl-1-T7-R | TAATACGACTCACTATAGGGTGTTCCTCATCGGCTTCT |
| Bx-cpl-2-T7-F | TAATACGACTCACTATAGGGACTAGATCCCAGCGCCACT |
| Bx-cpl-2-R | AGCCAACAGTCACGACAGC |
| Bx-cpl-2-F | ACTAGATCCCAGCGCCACT |
| Bx-cpl-2-T7-R | TAATACGACTCACTATAGGGAGCCAACAGTCACGACAGC |
| Bx-cpl-3-T7-F | TAATACGACTCACTATAGGGAGAGCTTCACAGCAGTGCCAAG |
| Bx-cpl-3-R | GTTGAACCTGGTAACTATAGTC |
| Bx-cpl-3-F | GCTTCACAGCAGTGCCAAG |
| Bx-cpl-3-T7-R | TAATACGACTCACTATAGGGAGAGTTGAACCTGGTAACTATAGTC |
| gfp-T7-F | TAATACGACTCACTATAGGGAGACCATGGCCAACACTTGT |
| gfp-R | AGATAATCCCAGCAGCAGTT |
| gfp-F | AGACCATGGCCAACACTTGT |
| gfp-T7-R | TAATACGACTCACTATAGGGAGATAATCCCAGCAGCAGTT |
| Real Time PCR | |
| q-Bx-cpl-1-F | CCAGAAGCCGATGAGGAACA |
| q-Bx-cpl-1-R | CCAGTTTTGTAGAGTTGGAAGC |
| q-Bx-cpl-2-F | AGTCATCGCTGTAATCTGC |
| q-Bx-cpl-2-R | TTGTTGGTGCCATAAGTG |
| q-Bx-cpl-3-F | CTATAACGGAGTCACCTCCAT |
| q-Bx-cpl-3-R | TGCTCTTCACTGAGATCCAGT |
| Actin-F | GCAACACGGAGTTCGTTGTAGA |
| Actin-R | GTATCGTCACCAACTGGGATGA |