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. 2018 Dec 21;20(1):19. doi: 10.3390/ijms20010019

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Minimum or no surface expression of Siglec-8 on leukocytes other than mast cells and basophils. Splenocytes were collected from WT and CPA3-Siglec-8 mice and analyzed for Siglec-8 expression after gating to CD45⁺ and specific cell markers, CD3 for T cells, CD19 for B cells, CD11c for dendritic cells (DC), Gr-1 for neutrophils and monocytes, Siglec-F for eosinophils, and CD11b for macrophages. The numbers are percentages of indicated cell populations. Shown are representative plots of three independent experiments.