Figure 7.

A subset of BMMCs from Mcpt5-Siglec-8 mice express high levels of surface Siglec-8. BMMCs were generated from Mcpt5-Siglec-8 (n = 4) and control (Mcpt5-Cre^−/− SIG8^+/−, n = 5) mice. (A) Mcpt5 and (B) Siglec-8 mRNA levels were quantified by qPCR weekly during the maturation of the mast cells; (C) purity of BMMCs was assessed weekly by flow cytometry showing % FcεRI⁺ c-Kit⁺ of live cells; (D) surface Siglec-8 protein expression was determined by flow cytometry; (E) representative flow cytometry plots of BMMCs (at four weeks) showing FcεRI⁺ c-Kit⁺ cells and gating for Siglec-8⁺ cells. Data are from three independent experiments, and the mean ± SEM of n = 4–5 are displayed.