Table 1.
Antibody Panel Information.
| Antibody | Clone | Fluorophore Conjugation | Source |
|---|---|---|---|
| Anti-Siglec-8 (mouse IgG1) | 2E2 | Alexa Fluor 488 | Produced in house |
| Mouse IgG1 Isotype | MOPC-21 | Unconjugated, Alexa Fluor 647 or Alexa Fluor 488 | Tonbo Biosciences, San Diego, CA, USA Conjugated to Alexa Fluor 488 in house |
| Anti-CD3 | 500A2 | Pacific Blue | Invitrogen |
| Anti-CD11b | M1/70 | APC-Cy7 | BD Pharmingen |
| Anti-CD11c | N418 | APC | Biolegend |
| Anti-CD19 | 1D3 | PE | BD Biosciences |
| Anti-CD45 | 1O4 | PE-Cy7 | BD Pharmingen |
| Anti-CD45 | 30-F11 | APC-Cy7 | BD Pharmingen |
| Anti-CD49b | DX5 | PE | BD Pharmingen |
| Anti-CD117 (c-Kit) | 2B8 | BV421 | BD Pharmingen |
| Anti-CD117 (c-Kit) | 2B8 | APC | eBioscience |
| Anti-CD117 (c-Kit) | 2B8 | FITC | eBioscience |
| Anti-FcεRI | MAR-1 | PE-Cy7 | Biolegend |
| Anti-FcεRI | MAR-1 | PE-Cy7 | eBioscience |
| Anti-FcεRI | MAR-1 | APC | Biolegend |
| Anti-Ly-6G and Ly-6C (Gr-1) | RB6-8C5 | Pacific Blue | Invitrogen |
| Anti-Ly-6G and Ly-6C (Gr-1) | RB6-8C5 | eFluor450 | eBioscience |
| Anti-Siglec-F | E50-2440 | PE | BD Pharmingen |
| Anti-Siglec-8 (mouse IgG1) | 2C4 | Alexa Fluor 647 | Produced in house |
| Anti-Siglec-8 (mouse IgG1) | 7C9 | PE | Biolegend |
| Anti-Siglec-8 (mouse IgG1) | 7C9 | APC | Biolegend |