Figure 3.

Effect of compounds 2 and 3 against amastigotes of L. amazonensis. Macrophages from peritoneal exudate were collected from BALB/c mice and infected with L. amazonensis promastigotes in the stationary growth phase during 6 h. The cells were washed and incubated during 24 h without treatment. After this period, the macrophages were treated with the compounds at 6 µM for 48 h. (A) Percentage of infected cells was evaluated by counting of 100 cells as well as the parasite number. (B). p-value was measured by unpaired t-test compared to control group.