Figure 5.

Automated quantification of amastigotes after treatment. (A,B) Infected cells were treated with AMB at different concentrations. The automated image segmentation and parasites quantification workflow were applied to determine the percentage of infection as well as the number of amastigotes. Bars represent the mean ± SD of one representative assay performed with eight replicates. (C,D) Quantification of the biological response after the treatment with AMB and compound 2 at concentration ranging from 0.15 to 10 µM. The correlation coefficient (r2) is 0.97 for AMB and 0.65 for compound 2. Red dot means the logarithmic concentration related to 50% biologic response.