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. 2019 Jan 4;20(1):168. doi: 10.3390/ijms20010168

Figure 2.

Figure 2

mRNA (A) and protein (B) expression of matrix metalloproteinases in chondrifying micromass cultures. Optical densities of signals were measured and results were normalized to the optical densities of 0-day cultures. In panels (A,B), the numbers below the signals represent integrated densities of signals determined by ImageJ freeware. For RT-PCR and Western blot reactions, GAPDH (A) and actin (B) were used as internal controls. Zymography (C) with collagen type I, gelatin, and casein substrates was also performed. Signals for MMP9 at 75 kDa, MMP13 at 54 kDa, proMMP9 at 85 kDa, and MMP1 at 54 kDa are labeled by arrows. Densities of three independent experiments are shown in the figures. Asterisks indicate significant differences compared to the 0-day cultures (* p < 0.05, one-way ANOVA followed by Tukey’s HSD test).