Table 1.
Nucleotide sequences, amplification sites, GenBank accession numbers, amplimer sizes, and polymerase chain reaction(PCR) reaction conditions for each primer pair are shown.
| Gene | Primer | Nucleotide Sequence (5’→3’) | GenBank ID | Annealing Temperature | Amplimer Size (bp) |
|---|---|---|---|---|---|
| Admats4 | sense | GTG GCA AGT ATT GTG AGG G(2079–2097) | NM_172845.3 | 54 °C | 124 |
| antisense | AGG TCG GTT CGG TGG TT(2186–2202) | ||||
| Hyal1 | sense | GGG GTC TTT GAT GTC GTG G(371–389) | XM_015292793.1 | 57 °C | 486 |
| antisense | CGG GTC GCT GAA GTT GTT(839–856) | ||||
| Hyal2 | sense | ACA ACC ACG ACT ACA GCA AGA A(784–805) | XM_414258.5 | 56 °C | 452 |
| antisense | CGC TGC CAT CGT CAC ATT(1218–1235) | ||||
| Hyal3 | sense | TAC GGC ATC GTG GAG AAC CG(265–284) | XM_003641994.3 | 59 °C | 450 |
| antisense | CCA GTC GTC GTT GAA GCA GTC G(693–714) | ||||
| Hyal4 | sense | CCA CCG TGC CTT GCT ATT(325–342) | XM_017011911.1 | 51 °C | 254 |
| antisense | GTT TGC TGC TGG TCC TTT(560–577) | ||||
| MMP1 | sense | TTT GTG ACC CTA ACT TGA(1021–1038) | XM_417176.4 | 47 °C | 473 |
| antisense | GAC ATA GCC ATC TTT CTG(1476–1493) | ||||
| MMP7 | sense | AAA AGA GTT ACC TCG GGA CA(483–502) | NM_007742.3 | 52 °C | 250 |
| antisense | CAC GGA CAT TTG AGT GGG(717–735) | ||||
| MMP8 | sense | TGT CAA GGG CTG AAG TGA(478–495) | NM_008611.4 | 51 °C | 382 |
| antisense | TGA GGT AGT GAA TAG GTG C(841–859) | ||||
| MMP9 | sense | TTC TGG ACT CTG GGA ACC G(625–643) | NM_204667.1 | 57 °C | 237 |
| antisense | GGG AGA CCC ATC GCT GTT(844–861) | ||||
| MMP13 | sense | CAT GCA GAA ACC ACG ATG(296–313) | NM_001293090.1 | 51 °C | 395 |
| antisense | GAG CAG CAA CAA GAA ACA AG(671–690) | ||||
| GAPDH | sense | GAG AAC GGG AAA CTT GTC AT(238–258) | NM_204305 | 54 °C | 556 |
| antisense | GGC AGG TCA GGT CAA CAA(775–793) |