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. 2019 Jan 4;20(1):168. doi: 10.3390/ijms20010168

Table 1.

Nucleotide sequences, amplification sites, GenBank accession numbers, amplimer sizes, and polymerase chain reaction(PCR) reaction conditions for each primer pair are shown.

Gene Primer Nucleotide Sequence (5’→3’) GenBank ID Annealing Temperature Amplimer Size (bp)
Admats4 sense GTG GCA AGT ATT GTG AGG G(2079–2097) NM_172845.3 54 °C 124
antisense AGG TCG GTT CGG TGG TT(2186–2202)
Hyal1 sense GGG GTC TTT GAT GTC GTG G(371–389) XM_015292793.1 57 °C 486
antisense CGG GTC GCT GAA GTT GTT(839–856)
Hyal2 sense ACA ACC ACG ACT ACA GCA AGA A(784–805) XM_414258.5 56 °C 452
antisense CGC TGC CAT CGT CAC ATT(1218–1235)
Hyal3 sense TAC GGC ATC GTG GAG AAC CG(265–284) XM_003641994.3 59 °C 450
antisense CCA GTC GTC GTT GAA GCA GTC G(693–714)
Hyal4 sense CCA CCG TGC CTT GCT ATT(325–342) XM_017011911.1 51 °C 254
antisense GTT TGC TGC TGG TCC TTT(560–577)
MMP1 sense TTT GTG ACC CTA ACT TGA(1021–1038) XM_417176.4 47 °C 473
antisense GAC ATA GCC ATC TTT CTG(1476–1493)
MMP7 sense AAA AGA GTT ACC TCG GGA CA(483–502) NM_007742.3 52 °C 250
antisense CAC GGA CAT TTG AGT GGG(717–735)
MMP8 sense TGT CAA GGG CTG AAG TGA(478–495) NM_008611.4 51 °C 382
antisense TGA GGT AGT GAA TAG GTG C(841–859)
MMP9 sense TTC TGG ACT CTG GGA ACC G(625–643) NM_204667.1 57 °C 237
antisense GGG AGA CCC ATC GCT GTT(844–861)
MMP13 sense CAT GCA GAA ACC ACG ATG(296–313) NM_001293090.1 51 °C 395
antisense GAG CAG CAA CAA GAA ACA AG(671–690)
GAPDH sense GAG AAC GGG AAA CTT GTC AT(238–258) NM_204305 54 °C 556
antisense GGC AGG TCA GGT CAA CAA(775–793)