Figure 1.

Frequencies of homoplasmic point mutations in the whole mtDNA of immortalized human breast cells. Error bars represent the Wilson score 95% confidence intervals. (A) Overall frequencies determined by performing conventional NGS, SSCS, and DCS analyses; (B) Homoplasmic mutation frequency of specific mutation types determined using conventional NGS (representative data). Same pattern of frequencies of specific mutation types is observed for all experiments regardless of the sequence method used.