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Frequencies of rare point mutations in the whole mtDNA of immortalized human breast cells. Error bars represent the Wilson score 95% confidence intervals; (A–C) Overall frequencies determined by performing conventional NGS, SSCS, and DCS analyses. Rare mutation frequency of each mutation type as determined using (D–G) conventional NGS, (H,I) SSCS, and (J,K) DCS analyses. Error bars represent the Wilson score 95% confidence intervals.
