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. 2019 Jan 4;20(1):163. doi: 10.3390/ijms20010163

Figure 2.

Figure 2

RSU-1 depletion from MCF-7 and MDA-MB-231 cells leads to downregulation of Growth Differentiation Factor-15 (GDF-15) at the mRNA and protein level. (a) Relative GDF-15 mRNA expression in MCF-7 and MDA-MB-231 cells transfected with NSC or RSU-1 siRNA for at least 48 h. Six (6) independent real-time PCR experiments were performed, and data were analyzed using the ΔΔCt method and having NSC-treated cells as calibrators. Asterisks indicate statistically significant changes (p-value < 0.05). (b) Representative immunoblot showing GDF-15 protein expression in both BC cell lines treated with NSC or RSU-1 siRNA for at least 48 h. B-actin was utilized as loading control.