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. 2018 Dec 29;24(1):110. doi: 10.3390/molecules24010110

Figure 3.

Figure 3

Effects of SHK on cell viability and oxidative stress in L-02 cells. (A) L-02 cells were treated with increasing concentrations of SHK (0.25, 0.375 and 0.50 μM) for 1 h, then use APAP (10 mM), cultured for 24 h, and then cell viability was evaluated by MTT assay. (B,C) L-02 cells were treated with SHK for 1 h, then treated with APAP (10 mM) and cultured for 8 h together, and then GSH and ROS was assessed using a commercial GSH test kit and 2houghdichlorofluorescin diacetate (DCF-DA) fluorescence assay, respectively. All of the data represent the average from three independent experiments. * p < 0.05 versus the control group; # p < 0.05 and ## p < 0.01 versus the APAP group.